进化毒理学——化学品管理的信息工具？

进化毒理学关注的是污染驱动的种群间遗传分化的驱动因子,机制和结果。焦点问题涉及作为选择压力的化学污染类型,遗传学、表观遗传学和受种群影响的人口统计学,以及可能伴随着快速适应的适宜性代价和交叉抗性。在这一领域,研究人员将环境化学、保护遗传学、种群生物学和毒理学的工具结合起来,以了解受影响群体的稳定性和健康。最近有关进化毒理学的研究展示了多样化的种群适应污染案例（青鳉鱼、绦钩虾属和食蚊鱼）。根据定义,“适应”是通过某些个体和基因型的局部丧失实现的,因此提供了生物多样性丧失的单一证据。化学品管理通常主要通过实验室研究预测评估与化学品暴露相关的风险,进化毒理学则通过提供对种群和群落的影响的直接证据补充了这种方法。最近由环境毒理学和化学学会（SETAC）工作组EVOGENERATE（进化和化学品的多代效应）发起了讨论进化毒理学研究对化学品管理的效用。为了进一步讨论,请学术界、政府和工业界三个部门各派一名代表就下列问题发表意见：[在正文中提供]     

Stressed, but not defenceless: no obvious influence of irradiation levels on antifeeding and antifouling defences of tropical macroalgae

The production of defence metabolites is assumed to be costly in metabolic terms. If this holds true, low-light stress should reduce the ability of seaweeds to defend themselves chemically against herbivory and fouling. We investigated the effect of energy limitation on the defensive status of seaweeds by assessing their attractiveness to mesograzers and their activity against a bivalve macrofouler in comparison with non-stressed conspecifics. The macroalgae Codium decorticatum (Woodw.) M. Howe, Osmundaria obtusiloba (C. Agardh) R. E. Norris, Pterocladiella capillacea (S. G. Gmel.) Santel. and Hommer., Sargassum vulgare C. Agardh and Stypopodium zonale (Lamour.) Papenf. collected at the southeastern Brazilian coast were exposed to six levels of irradiation (between 1 and 180 μmol photons m⁻² s⁻¹) for 10–14 days. After this period, algae from all treatment levels were: (a) processed as artificial food and offered to an amphipod community dominated by Elasmopus brasiliensis Dana and (b) extracted to test for differences in settlement rates of the fouling mussel Perna perna L. on filter paper loaded with the crude extracts. Generally, photosynthesis rates and growth were reduced under low light conditions. Attractiveness to herbivores and macrofoulers, however, was insensitive to energy limitation. We discuss possible explanations for the observed absence of a relationship between light availability and algal defence including the change in nutritional value of the algal tissue, the allocation of resources towards defence instead of growth and the absence of costs for defence.     

The suitability of Macadamia and Juglans for cultivation in Nepal: an assessment based on spatial probability modelling using climate scenarios and in situ data

Regional Environmental Change - Global climate models predict temperature rises and changes in precipitation regimes that will shift regional climate zones and influence the viability of...     

Alpha-fetoprotein and acetylcholinesterase are not predictive of fetal junctional epidermolysis bullosa,Herlitz variant

Junctional epidermolysis bullosa, Herlitz variant (junctional EB-Herlitz) is a lethal autosomal recessive skin disorder currently amenable to prenatal diagnosis only by direct analysis of fetal skin. However, elevated levels of alpha-fetoprotein, as well as the presence of acetylcholinesterase in amniotic fluid, have been associated with other severe fetal genodermatoses. Fetal skin samplings were performed in ten pregnancies at risk for fetal junctional EB-Herlitz, with three fetuses affected on the basis of electron microscopic detection of blisters within the lamina lucida and abnormal hemidesmosomes. In neither affected nor unaffected pregnancies were maternal serum or amniotic fluid alpha-fetoprotein levels elevated. Moreover, alphafetoprotein levels in both maternal serum and amniotic fluid were not statistically different comparing affected and unaffected fetuses. Acetylcholinesterase was not present in the amniotic fluid samples of the three affected pregnancies. Unlike other severe fetal genodermatoses, neither alpha-fetoprotein nor acetylcholinesterase was predictive of junctional EB-Herlitz.     

Ultrasound-assisted emulsification-microextraction of fragrance allergens in water

A method based on ultrasound-assisted emulsification-microextraction (USAEME) and gas chromatography-mass spectrometry (GC-MS) has been developed for the analysis of regulated fragrance allergens in water. Extraction conditions such as the type of solvent, extraction temperature, irradiation time, and salting-out effect were optimized using a multivariate approach. Compounds were extracted during 2 min in an acoustically emulsified media formed by 100 μL chloroform and 10 mL sample. The USAEME process provided an efficient and exhaustive extraction (enrichment factor ～100) and, after centrifugation, the extract was ready for GC analysis. Validation was performed using spiked ultrapure water as well as other most complex matrices such as sewage water. Recoveries between 75% and 110% were generally obtained, and precision was characterized by RSD values <10% in most cases. The limits of detection (LODs) were at the sub-nanogram per millilitre level. The proposed procedure was applied to the determination of allergens in several real samples including tap water, baby bathwater, recreational place water, public washing place water, and sewage water. The presence of some of the target compounds was confirmed in all the samples excluding tap water, demonstrating the ubiquity of this group of cosmetic and personal care products ingredients.     

Simultaneous determination of chlorothalonil and its metabolite 4-hydroxychlorothalonil in greenhouse air: dissipation process of chlorothalonil

An analytical method was developed and tested for the simultaneous determination of chlorothalonil and its main metabolite 4-hydroxychlorothalonil, in airborne samples. High performance liquid chromatography equipped with Ultra-violet detector was used to separate and quantify the analytes. Glass microfibre filters for the collection of the analytes' particles were tested. Solid sorbents, such as Tenax, Florisil, XAD-2 and silica gel, were studied to find out the most suitable material for the collection of the analytes in the gas phase. The results have shown that only chlorothalonil was trapped in the vapor phase with highest results obtained when silica gel was the sorbent of choice. Linearity was demonstrated in a wide concentration range 0.01-10.00 mg L(-1). Recoveries from spiked glass microfibre filters and silica gel cartridges for chlorothalonil and 4-hydroxychlorothalonil were almost quantitative. The quantification limits were calculated to be 8.4 and 19.6 ng m(-3) in air for chlorothalonil and 4-hydroxychlorothalonil, respectively. The two analytes spiked on the GF/A filters and silica gel cartridges were proven to be stable for more than 15 days, at 4degrees C and ambient temperature. The applicability of the present method was demonstrated by the analysis of the chlorothalonil and its metabolite in greenhouse air.     

Fertility of female mice fed coumestrol and diethylstilbestrol

Abstract

Coumestrol, a compound produced by various legumes which exerts estrogen‐like activity in animals, and diethylstilbestrol (DES) were studied as chemical agents for controlling reproduction in mice. Female mice were fed control diets or diets containing 100 ppm coumestrol for eight days. Female mice were exposed to males and reproductive tracts examined 14 days later. Litter size was not affected by 100 ppm dietary coumestrol but feed consumption was reduced 17%. Similar trials were conducted with mice fed 1 ppm DES. Vaginal plugs were present in 50% of the females fed 1 ppm DES, but no fetal pups were present. Feed intake was reduced 37% by the DES. Levels of 0, .1, .25, .50, .75 and 1.0 ppm DES were compared in two strains of mice, Swiss and ICR. Reproduction in both strains was totally inhibited by all DES treatments. The use of DES to control rodent populations warrants further investigation.     

GIS and multiple-criteria evaluation for the optimisation of tsetse fly eradication programmes

Tsetse flies are the vectors of trypanosomes, the causal agent of trypanosomiasis, a widespread disease of livestock and people in Africa. Control of tsetse may open vast areas of land to livestock-keeping, with the associated benefits of developing mixed crop-livestock production systems. However, as well as possible positive impacts there are also risks: bush clearing would accelerate and cattle numbers would rise, leading to a reduction of vegetation cover, and an increase in runoff and erosion; there may also be increased pressure on conserved areas and reductions in biodiversity. The objective of this study is to show how remotely sensed and other environmental data can be combined in a decision support system to help inform tsetse control programmes in a manner that could be used to limit possible detrimental effects of tsetse control. For Zambia, a methodology is developed that combines a tree-based decision-support approach with the use of Multiple-Criteria Evaluation (MCE), within a Geographical Information System (GIS), in order to target areas for tsetse control. The results show clear differentiation of priority areas under a series of hypothetical scenarios, and some areas (e.g. northwest of Petauke in the Eastern Province of Zambia) are consistently flagged as high priority for control. It is also demonstrated that priority areas do not comprise isolated tsetse populations, meaning that disease control using an integrated approach is likely to be more economically viable than local eradication.